Using your own experience married with the research.
NOT more than 06 Hours
4 hours a day
Thanks Yao Doe and Brig Singh for responses.
At least two hours
AGN (Active Galactic Nuclei) are high energy galaxies powered by supermassive blackholes. The evolution of a galaxy is characterized by parameters such as luminosity and redshift. The redshift is...
25 June 2024 3,782 2 View
Thermal emission is temperature dependent. Non-thermal emission is not temperature dependent such as synchrotron emission.
29 May 2024 5,155 2 View
Investigate the socio-economic status of women in the Western Cape, including factors such as labour force participation, wage gaps, access to education and healthcare, and representation in...
25 March 2024 7,972 1 View
Good day everyone, Please advise me. I always wondered if one of the benefits of EQ is emotional well-being? OR Is emotional well-being a different subject on its own? I am also trying to...
23 July 2022 336 3 View
I have linked Arlequin to rcmd, and even downgraded my version of R to 3.0.1, however the rmcd button results in the black panel flashing for a second or two and no produced images in the Graphics...
23 July 2022 5,967 1 View
I have been using DNASP to run analysis on my sequences, but with linkage disequilibrium the analysis does not run and I get the message in error(2).
21 July 2022 8,537 0 View
I have agarose gel slices with PCR products in them for purification and sequencing. I need to send the slices to another lab for processing which may take up to 5 working days. Is it okay to...
03 July 2022 779 4 View
I am using nested PCR to determine the alleles present in my parasite population for a specific gene. This has gone well, but one gene has given me an issue. The initial PCR for gene MSP1...
27 June 2022 8,942 3 View
I have sequenced purified amplicons by Sanger sequencing. I want to create a consensus sequence contig for each amplicon. Using the .ab1 files I trimmed the ends of the forward and reverse...
16 April 2022 6,558 6 View
I have outsourced Sanger sequencing of my purified PCR products. Looking at the chromatogram, I know to trim the ends. However, the ends that need to be trimmed according to the quality scores are...
07 April 2022 7,629 3 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...
31 July 2024 2,150 0 View
EDX analisys
31 July 2024 5,947 2 View
I am working on algal extract to which gas chromatography (Not GC-MS) spectrum I want to discover. My question is can we identify specific compounds using retention time if I compared the RT with...
29 July 2024 8,034 4 View
I have been running a MAGeCK test command on the terminal for a CRISPR screen to rank sgRNAs and genes based on the read count tables. However, I get only the plot of the top-ranked genes...
28 July 2024 9,811 1 View
if you have MRS broth already prepared, what is the manner you will follow for preparing hyper-sweet medium for screening of EPS producer LAB? what the concentration of saccharose or glucose you...
26 July 2024 6,606 0 View
"Integration of Mass Media and Communication into the System of International Relations: Place, Role, Actuality and Prospects: Modern Media Issues 2024" Website: https://immcir.alnoor.edu.iq/
23 July 2024 3,139 6 View
Why methanol and sulphuric acid, used in the analysis of polyhydroxyalkanoates (PHA) methyl esters by GC-MS? Additionally, why do we typically use non-polar solvents in GC-MS?
22 July 2024 1,210 2 View