How much time is needed to complete a master's thesis using CRISPR/Cas9?

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which material is used as base for thin film sample preparation in XRD analysis?

kindly guide me to prepare a Thin-film sample of nanoparticles for XRD analysis.

10 January 2021 3,744 4 View

Why is Statement of Problem one of the most challenging part of academic research? Why do supervisors pay more attention to it instead of methodology?

Some MSc and Ph.D. supervisors are having a lot of issues with understanding the Problem Statement of their students' research works. Sometimes, it leads to complete rejection of the whole...

04 January 2021 3,574 6 View

Anyone knows about .odb_f file generated by ABAQUS?

Hello all, I'm running a Bone Remodeling simulation in ABAQUS in which I just use basic ABAQUS Static simulations in every loop with an amplitude table to model the loading behavior & after...

19 October 2020 4,417 2 View

How to apply distributed load in a 2D model with plane stress/plane strain thickness in ABAQUS?

Hello all, I'm currently working on setting-up a 2D bone remodeling problem of a femur from literature. I take a 2D femur geometry and give it an out-of plane plane stress/plane thickness in...

06 October 2020 2,623 5 View

Is it possible to get a standard questionnaire or how to develop it, to measure perception & attitude of healthcare professionals towards investment?

I'm planning to adopted a standard questionnaire to conduct a survey that will measure the perception and attitude of healthcare professionals on investment and access to loans in the...

20 September 2020 4,911 2 View

In case of nanofluid flow within an enclosure, does the heat transfer trend follow the same trend as the thermal diffusivity of the nanoparticles?

I am trying to simulate the nanofluid flow within an enclosure for different nanoparticles e.g. Ag, Cu, CuO, Al2O3 and TiO2 . In the case of Ag and Cu, the heat transfer rate are almost same. I...

04 September 2020 7,988 7 View

Does Recycled aggregate offer strength?

Construction and demolition waste are increasing in large amount and now it is employed as a replacement of coarse aggregate in concrete, use of these type of waste do offer strength in concrete...

07 June 2020 4,548 11 View

Can I use flow cytometry to detect a rare population of around 150 cells in a single-cell suspension of 150,000 - 500,000 cells?

I am looking to detect a rare population of 150 cells via flow cytometry and analyze a subset of this population with one cell-surface marker. Sample size will be about 150,000 to 500,000 cells in...

12 May 2020 8,336 7 View

How to evaluate the In vitro study for coronavirus (COVID 19 Inhibitory activity)?

Dear Researchers .... Need help for COVID 19 drug discovery. Recently, COVID-19 main protease crystal structure was deposited in the protein database (PDB ID: 6LU7...

08 March 2020 3,853 4 View

Please, I need clarification on the quality status of a journal listed in WoS Zoological Record. Is this index comparable to SSI, ESCI, etc?

So many journals claim they are indexed in WoS (ISI in old parlance; now called Clarivate Analytics). However, most of the journal end up listed in the Zoological Record (ZR). Reading about that...

03 March 2020 1,733 3 View

In what ways do fellow reaerchers think that mRNA vaccination may be under CRISPR systems technology application ?

There are many ways for mRNA to intervene with genome expression. This question is raised for experts in CRISPR systems. Any RNA does not stand alone within acell. There are many ways to become...

18 February 2021 9,109 5 View

How can I completely delete a protein on the genomic level using Crispr?

I want to completely delete or largely truncate a gene and not only achieve a functional knockout in a cell line. I can only transfect my cells with the Crispr/Cas9 sgRNA RNP and not introduce a...

08 February 2021 2,032 2 View

Choise of cell line for neurobiology?

I am working with myelin-related proteins and interested to do cell experiments with my target protein in cells. I have created lentiviruses for my proteins, and mutants and also CRISPR set-up for...

02 February 2021 8,083 1 View

Does gene insertion via CRISPR/Cas9 result in chromosomal abnormalities?

Hello, I hope to insert a gene into mammalian cells in culture using CRISPR/Cas9 and an HDR template. Will insertion of a 1 kilobase gene cause chromosomal abnormalities and effect cell...

31 January 2021 2,516 1 View

Does anyone has a protocol for transfecting AML12 cells?

I am optimizing a CRISPR/CAS9 system in vitro before using it in vivo. I would like to transfect AML12 cells with my pAAV-sgRNA-vector to validate editing efficiency before generating virus.

26 January 2021 8,733 2 View

Triggering feeding of beetles / how to make them hungry?

Hey, I am working with Psylliodes chrysocephala feeding on oilseed rape. In feeding trials I often have the problem that a huge portion of the beetles do not feed at all. In clip cages I observed...

19 January 2021 9,851 6 View

Should I add a promoter upstream of my CRISPR-inserted gene?

Hello, I want to insert a gene into my cells using sgRNA, Cas9, and an HDR template. The gene that I will insert into has its own promoter. Because I will cut the gene downstream of its start...

14 January 2021 1,170 2 View

Does anyone have problems performing CRISPR KO in human primary T cells?

For the past few months, I have been troubleshooting a CRISPR KO of human CD3e in human primary T cells. I got the KO to work one time using 1250 ng TrueCut Cas9 (Thermo) + 250 ng (or 7.5 pmol)...

11 January 2021 6,016 3 View

After CRISPR and single-colony picking, cells reverted to WT?

Hi guys: I have been doing CRISPR knock-in and knock-out and one seemingly impossible scenario keeps happening. In the CRISPR knock-in attempt I tried to add a tag on a gene of interest, so I...

07 January 2021 190 3 View

How to choose mouse cell line to work on?

Hey, I am looking to start working on mouse cell line for some genomics experiments. I have the following requirements: 1) Normal chromosomes composition. 2) Possible to transfect. 3) Possible to...

03 January 2021 4,387 2 View

Ajay Keot

It will depend. If you know which gene to KO in that case you should keep at least one year for the safe side (till T0 line analysis). if your work involves analysis in T1 or more generations than more time.

Sukanta Bala

Ajay Keot thanks for your answer, will analysis the T0 for thesis, as before graduation I need to publish it in a SCI journal.

Kourosh Vahdati

It's better you do CRISPR/Cas9 for your PhD thesis. For more info contact Dr Masoud Tohidfar

Kourosh Vahdati Thank you for your comment. Mainly my thesis will be on CRISPR/ Cas9 , because of the border restriction in China I am bit concerned about the time I will get.