Can anyone suggest why my PCR results gave big differences in the control group?

I did RT-PCR using SYBR Green and used 2 ul of cDNA per sample to investigate the effect of LPS in primary osteoclast culture. I loaded the same amount of RNA to make cDNA (550ng/sample). However...

17 September 2020 9,193 3 View

Dear, why in HPLC method with detector UV-Vis not appear peak in chromatogram in wavelength maximum?

In my research, to check purity of my synthesized compound I use HPLC method with detector UV-Vis. So, first I did spectroscopy UV my compound and appear two peaks maximum (237 & 367 nm) ....

15 May 2016 6,989 8 View

Can anyone help, why my GAPDH band seems not uniform?

I make cDNA from uniform RNA concentration 1 mikrogram/mikroliter, did it have corelation with PCR condition?? or because my RNA have low quality??

20 October 2015 7,156 2 View

Is this the vesicula seminalis or uterus masculinus?

I am studying the "Guinea-Pig" male reproductive system. After dissection, I found a structure (picture below) that is explained differently in various places in the literature. Some claimed it...

07 November 2014 5,106 19 View

Can anyone tell me what kind of cell is this?

I found this cells (picture below) when i did hemocytometry of Cavia sp.'s blood with Turck reagent. But it can't be found in smear preparation with Giemsa staining. It was big ovoid cell, central...

11 May 2014 8,181 10 View

Can anyone advise on Gravity Model : Country-time Dummies and Specification Error?

Currently, I am doing research about the impact of free trade agreements to trade flow. I have panel data that consist of 86 countries from 1980-2012. I use panel data estimation : Pooled Least...

23 April 2014 9,811 3 View

Any advice on MDweb warning while checking protein?

I am using MDweb to analyse MD. While checking protein, I have been given some warning about polar acceptor clashes, apolar clashes and ionic negative clashes. Any suggestions?

30 November 2013 1,534 1 View

Does anybody know a web server that is running Molecular Dynamics (MD)?

I want to do a simulation with Molecular Dynamics but lack the facilities.

30 November 2013 9,352 16 View

What are some tricks to modelling large proteins using quark?

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03 November 2013 9,298 5 View

Protein modelling - can anyone help?

I need to model the IRS-I protein, but the homology modelling and I tasser result is not good enough, I have planned to use quark, but protein length is too large for quark.

02 November 2013 7,700 8 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View