How much tetracycline should I use to activate my gene of interest in a Tet-inducible system?

12 August 2024 1 2K Report

Hello,

I transduced gastric cancer cell lines with Tet-inducible plasmid that have my gene of interest. Now, I need to find the concentration and duration of tetracycline treatment that can activate my gene of interest. What range of concentration and duration do you suggest? (I have three kinds of plasmids: overexpression, knockdown and control (backbone) of my gene of interest).

Lara Pacheco

Hi Fariba,

I'm in the same situation that you and found this protocol online: https://www.takarabio.com/documents/User%20Manual/Tet/Tet-One%20Inducible%20Expression%20System%20User%20Manual.pdf

Maybe will help you,

Kind regards,

Lara

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