Lara Pacheco

2 Questions 2 Answers 0 Followers

Questions related from Lara Pacheco

Optimal Protocol for Transfecting IVT gRNAs into dCas9-Expressing iPSCs and Primary Fibroblasts?

I have iPSC and primary fibroblast lines that constitutively express dCas9, and I am planning to screen RNA guides produced by IVT in these cells. Since the cells already express dCas9, I only...

03 September 2024 9,482 0 View

All my transfected cell die after puromycin selection, wht to do?

Hello all, I want to immortalize human adult fibroblasts with a lentivirus plasmid containing hTERT under the promoter EF1A and puromycin resistance under the promotor mPGK. I performed a...

15 May 2024 7,004 3 View