How much TCID50 should I use to challenge immunized mice and thus effectively evaluate a vaccine candidate?

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Arturo Liñan

PhD. Guillermo Posadas-Herrera thanks a lot for helping me. Now I can understand what I have to do.

Vladimir Vonka

The dose used for challenge shoud be based on the virus titre in mice not in tissue culture It is a long tradition that 100 ID50 are.being used,

Brahimi Mahfoud

During the challenge using mice there are two parameters :

The number of LD 50 to inject and under what volume ? The volume should be small to avoid volume-related accidents especially for the I.C. route. To avoid this, prepare viral suspension with high-titre of virus challenge.

Thanks Vladimir Vonka and Brahimi Mahfoud