How much of the feed ration of Israeli dairy cows is imported?

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Anyone with some Transgenomic Wavemaker software? Seeking help from the chromatography community?

I am trying to seek out some Transgenomic Wavemaker software (happy to purchase a computer too) to operate a fraction collector. The only way to connect to the fraction collector so far is to...

23 February 2021 9,181 1 View

Best way to optimize species-specific primers? Can I use a temp gradient to test a Ta where I will get target (and no non-target) amplification?

Hello, I am testing different species-specific primer sets. I have two targets (two species, but same gene target). I am trying to select the best primers -- one that will amplify the target...

18 January 2021 1,981 6 View

I am trying to amplify my gene of interest (GOI, 2000 kb long) by PCR. My previously designed primers are not working. How can I optimize the primers?

As I wanted to amplify my GOI, I chose first 18 nucleotides including start codon in my forward primer. I add BAMHI recognition site at the beginning and 3 nucleotide extension for the efficient...

12 January 2021 3,327 3 View

Summer Studentship opportunities in Analytical Chemistry LC-MS HPLC Chromatography?

The ChromSoc/BMSS 2021 Summer Studentship Applications are now OPEN (DEADLINE 15th JAN). ChromSoc & BMSS , annually funds research projects to take place over the summer . The scheme provides...

17 December 2020 5,535 3 View

How do make a horizontal Boxplot incl. Outliers? (Excel, SPSS, others)?

I have recorded a time series (in minutes + seconds) and would like to make a horizontal boxplot. I have vertical boxplots in SPSS and Excel. However, Excel is not able to make horizontal boxplots...

30 November 2020 9,005 6 View

Has anyone generated Flp-In host cell lines from HeLa TRex cells? If so, anyone willing to share a detailed protocol ?

I am trying since 2 months to generate HeLa Flp-In TRex cell lines, but failed. If someone have a detailed protocol, including cell number to seed, transfection method used, antibiotic...

22 November 2020 5,723 3 View

I treated my fixated cells with PI for Cell Cycle Analysis but our Flow Cytometry did not work. How long can I keep my samples stable(+4) with PI dye?

I prepared my samples with ethanol, RNase and PI. However I could not use the Flow Cytometry machine because of an technical problem. I covered my samples with foil and put them in +4. As my...

21 November 2020 9,132 6 View

Statistics on prevalence of methodological approaches in psychological research?

I am currently looking for data on psychological research. I am interested in -current and previous- prevalence of methodological approaches in psychological research (e. g. the proportion of...

15 November 2020 5,711 3 View

Does Formic Acid linger in the MS?

Does anyone know if formic acid has a tendency to linger in the MS after organic solvent cleaning? If it does, are there any special processes that can be put in place to remove it? Thanks!

11 October 2020 3,828 2 View

SPSS frequencies bar chart?

Hello, is it possible to get a bar chart in SPSS where I have the frequencies on the y-axis and two variables on the x-axis? How do I get the two variables on the x-xis? thank you

01 October 2020 6,876 7 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

What are the most relevant MSc proposal topics for Petroleum Production Engineering at the moment?

I am a Petroleum Production Engg MSc student at Robert Gordon University in Aberdeen and I am in the process of selecting a topic for my MSc Engineering Investigation proposal. I would very much...

01 March 2021 7,396 4 View

Why is the sum of the elements in a row in a Q matrix equal to zero?

When explaining substitution models, the substitutions are expressed as Q matrix. Why is the sum of the elements in a row zero?

28 February 2021 3,864 3 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Does different dilution factors affect negatively on NH3 reading ?

I have a sample of treated water sample & asked to obtain ammonia conc. in sample using a machine with 5-25 ppm detection range. However, upon testing with different dilution factors, all...

27 February 2021 8,483 3 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

How to model Supercritical water gasification (SCWG) process in Homer software?

Hi everyone How to model Supercritical water gasification (SCWG) process in Homer software?

25 February 2021 4,075 1 View