How much is a strain when you purchase from JCM or ATCC?

More Princess Alyssa Abid's questions See All

Solution of 2D convection diffusion heat equation using MATLAB ?

This is a polymer heating problem. Polymer enters at around 180C (x=0) in the barrel. Also barrel initial temperature is 180 (t=0). Some heat Is added along whole length of barrel q. We have to...

15 February 2021 3,887 1 View

What happens if you accidentally stopped the western blot run for about one hour and then restart?

I am new to western blot and when I was running it today, the machine stopped the run by itself and I realized it only one hour after. The ladder was barely separated, so I restarted the run until...

17 January 2021 5,545 1 View

Paper about future and challenges of batteires?

I am looking for a recent paper that describes the challenges facing batteries' progress and all about the future of batteries.

11 January 2021 1,809 1 View

Recommendations for protein removal protocols in acidified EtOH?

I am trying to measure small molecule fluorescence in acidified ethanol brain lysate and need to remove protein, which is peaking around where my compound signal should be. Would a MWCO filtration...

03 January 2021 3,912 2 View

Is my math correct for protein reconstitution for western blot?

Hi! I'm brand new to western blots and still learning how to calculate the concentration and how to reconstitute lyophilized proteins. I have a 20ug vial I want to reconstitute, and we normally...

30 December 2020 9,819 3 View

For MTT assays, are biological replicates just multiple plates of the same set up and condition?

I am new to MTT assays and research in general. I am performing a MTT assay of the same cell line with 4 different conditions. How would you do biological replicates in this situation? I know I...

30 December 2020 1,730 3 View

Who to create single SPSS data sheet for different measures by two different populations??

I am working on Effect of parents personality patterns on adolescents, I have collected data from parents and adolescents by using different scales on both population. Now I have to create SPSS...

26 December 2020 7,238 3 View

Why is light pure energy?

light is pure because the light is the simplest electromagnetic construct but the energy of any object is also of the same kind.

20 December 2020 207 4 View

How to determine drug concentration for protein inhibition?

Hi! I am doing an experiment where I inhibit a specific protein with a drug and then measuring cell viability. This drug has been used in literature in similar studies. However, I am not sure how...

14 December 2020 4,281 2 View

How can I manually add co-authors when uploading a published work?

14 October 2020 721 3 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Have anyone checked before Cyclic voltammograms of intial ITO substrate and E-teck?

As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...

23 February 2021 3,175 3 View

Universal bacterial primers targeting a mRNA?

Hi, I'm looking for universal primers targeting a bacterial mRNA to quatify bacterial load by qPCR in a complexe mixture (eukaryote and prokaryote RNA). Ribosomal RNA being depleted, I absolutly...

22 February 2021 9,969 3 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

Could you please share your experience with me on the type of 96 wells plate for MIC determination: Treated Vs Untreated and Round-Vs Flat-bottomed?

I am working on the MIC determination of various antibacterial agents (both water-soluble and- insoluble) against S. aureus, MRSA, and S. pyogenes. Some guidelines recommend using round-bottomed...

17 February 2021 7,029 3 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View