Help regarding free-floating immunohistochemistry on mouse spleen?

Hello fellow researchers I am trying to stain cryostat-sectioned spleen tissue, using free-floating chromogenic immunohistochemistry. Unfortunately, I'm not able to detect any staining (MHC class...

13 November 2020 6,884 3 View

How much plant powder do I need for different concentration of two extracts (Aqueous and Ethanolic extract)?

Supposed I need three different concentration for two plant extracts (namely: Aqueous and Ethanolic extract), starting at 50mg/ml, then 100mg/ml then 150mg/ml. In what particular weight of plant...

31 March 2019 2,775 5 View

Does anyone have any experience running ERIC-PCR?

11 May 2016 526 39 View

How do I get the electrode surface area for CR2032?

I am trying to compute the Lithium-ion diffusion coefficient (DLi) based on the equation attached[1]. So how do I get the A; electrode surface? and also how to calculate the dE/dx part of the...

13 March 2016 4,458 3 View

Any papers that have specific gases and its composition in carbonization of coconut shell?

carbonization, coconut shell, gases, carbonizer

24 February 2016 2,412 3 View

Dissertation query, Q - method inquiry. currently running a pilot study and am falling short on the analysis. All info welcome?

Hi, I'm an Undergraduate student at the University of Chichester and I'm currently writing my dissertation. I have all my statements and my question, i have knowledge of exel and spss software...

04 January 2016 3,364 4 View

What factors affect the electrode polarization for lithium-ion batteries?

Surface area, particle size? What other possible factors reduces/increases Electrode Polarization? And how does Electrode Polarization affect affect rate-capability and battery performance?

08 December 2015 2,303 6 View

Who will assist me in developing a new theoretical concept on our oceans' formation - ?

Hello and thank you for welcoming me here. I am thrilled to announce that I seem to have developed an in itself seemingly "water-proof" raw theory on the formation of our (and any) planet's...

01 September 2015 2,035 6 View

Did someone try to ablate metallic cerium?

I want to ablate metallic cerium but i'm concerned about the inflammable nature of cerium. Any suggestions? Thank you!

24 August 2014 1,285 2 View

How do I perform an ELISA validation?

I am performing a (partial) validation of commercially available ELISA kit. I am using the kit on a previously untested matrix, so I wish to validate the assays Accuracy, intra-assay Precision and...

02 April 2014 8,905 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Samples to study circular dichroism (chirality)?

I want to build a low-cost polarimeter to study circular dichroism (chirality). What type of samples can I use? I am looking for samples that can be found outside the laboratory.

01 March 2021 9,101 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View

Suppose we have a banana seller cart consisting of 4 wheels, weighing about 300 kg. How much torque motors are needed to pull of push it electrically?

I am thinking of electrifying the street food carts while harvesting energy using solar panels on top.

22 February 2021 4,323 3 View

What are the LAB methodologies (procedures, equipment etc) for CO2 enrichment experiments to study crop responses?

I am doing a proposal for a research entitled 'GRAIN YIELD AND QUALITY RESPONSES OF WHEAT GENOTYPES UNDER CO2 ENRICHMENT AND EXTREME EVENTS' I will be using climate chamber and I would like to...

18 February 2021 6,494 4 View

Does anyone have any experience with the Motic EasyScan Pro Slide Scanner?

I am interested in purchasing a slide scanner for histology and was presented with the Motic EasyScan Pro but I have never heard of the company, nor know of anyone who has used the system. Can...

11 February 2021 2,416 2 View

Are there any differences between setting automatic or manual number of measurements in DLS?

When doing measurements in DLS (either size or zeta potential), I have the option to input an automatic number of measurements or to set it manually. My question is: is there a drawback in...

08 February 2021 9,423 6 View

What's support me on Simulink Matlab Model of Regenerative Braking in DC Traction ?

I have a thesis on Utilize the electrical power from Regenerative Braking in 750 Vdc Traction by simulation in the Program MATLAB Simulink. I made the Power Load flow from 24kV to Traction...

08 February 2021 2,532 1 View