The creation of colitis is then treated with probiotics
Hi, I want to start my cell culture to run a MTT assay , how can I sterilize my polymer?
15 December 2020 1,813 1 View
Hi there, I have been struggling with solving my chitosan folate coated nanoseleniums in dmem culture media sincr it is only soluble in acetic acid , and hardly soluble in basic media. What can I do?
19 November 2020 5,636 3 View
Hi, I have a 25 ml sample of chitosan coated nanoselenium with average diametere of 112 nm, considering the fact that chitosan after being kept in water media swelles, how can I send a sample for...
08 November 2020 6,859 3 View
Hi there I wanted to know if it is ok to add 2 or 3 drops of glycerol for suspending the nanoparticles in water before measuring the zeta potential?
26 October 2020 2,146 3 View
I'm running a MTT test for evaluation the effect of chitosan coated nanoselenium versus bare nanoseleniums, what are the controls for MTT assay? Should I test bare nanochitosans as well as...
27 September 2020 9,525 2 View
Hi, I have a simple question: When we freeze cells why we use 90% FBS and 10% DMSO? I know that DMSO is used for cell cryopreservation but I don't know why we use FBS instead of culture media?
28 January 2020 7,077 3 View
There are many methods for a drug to be used, such as oral methods (pills), anal methods, injections, etc. But when a new drug invented, how do scientists choose the best way to insert it into the...
16 October 2018 2,535 18 View
Hello everybody, I was wondering to know how we can remove the primer dimer from PCR product in a way that, the quality and sharpness of the desired band remains unchanged.
09 October 2017 9,219 9 View
18 April 2017 4,399 3 View
I synthesize Fe3o4 nanoparticles and then i modified it by Sio2, how can i make stable dispersion of this coreshell (Fe3o4@Sio2) in water? should i use any surfactant?or should i functionalized it...
14 December 2016 7,516 2 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...
01 March 2021 9,525 4 View
Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...
01 March 2021 6,953 1 View
I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...
01 March 2021 3,703 3 View
Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...
01 March 2021 9,938 3 View
When explaining substitution models, the substitutions are expressed as Q matrix. Why is the sum of the elements in a row zero?
28 February 2021 3,864 3 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
I have a sample of treated water sample & asked to obtain ammonia conc. in sample using a machine with 5-25 ppm detection range. However, upon testing with different dilution factors, all...
27 February 2021 8,483 3 View
Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...
26 February 2021 4,026 5 View
Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...
25 February 2021 155 2 View
