RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

How can I homogenize this data to make a meta-analysis?

Hi, I am doing a review and hopefully meta-analysis for my Master thesis but I have the following problems: - Some studies only give the difference between end trial and baseline data and other...

22 February 2021 1,115 4 View

Research Gate Does it help researches to publicize their scientific work ?

In my opinion, the RG site is useful in solving scientific problems and shows the researcher's ability. Besides, the separation of the H-index from the self-citations and the overall index is an...

22 February 2021 3,894 6 View

Monte Carlo simulation?

Hi, i am conducting a Monte Carlo simulation for my new distribution but the MSE values are increasing while the sample size increase, does any experience this issue before and what should i do...

22 February 2021 3,680 4 View

Which is the best software or programming language (user friendly) for analysing data?

I have a basic knowledge on Python, R and MATLAB in analysing data. Among those, which is the best and user friendly? Which one should I learn deeply to analyse data?N:B: I want to learn data...

20 February 2021 3,950 15 View

Is There are any idea to achieve H NMR signals for swollen compound ??

the samples are not completely soluble - just swelled the solvents that's mean the sample may be partially soluble any suggestion, please

16 February 2021 8,801 3 View

How many samples can be pooled together for NGS?

We collected faecal samples from various species that we want to analyse their microbial communities' DNA via Next-Generation Sequencing. The problem is that due to budgetary constraints, we will...

16 February 2021 5,466 4 View

Flexibility of RNAseq?

When you use RNAseq I understand that it must follow certain conditions when you use it. One of them is the insertion of a polyA tail. The insertion of the polyA must include then...

05 February 2021 3,770 4 View

How to identify when a gaussian program is about terminate normally?

Dear all, I am trying to optimize a bimetallic complex using # opt=tight b3lyp/lanl2dz scf=qc geom=connectivity. The program is running for around 5 days but the size of the .chk remains same...

05 February 2021 8,721 3 View

Tick-borne encephalitis (TBE) incidence in the Czech Republic in recent years?

Dear Sir or Madam, i'm a medical student. In range of school lesson i have to research about TBE (see topic) in the country of Czech Republic. I would be pleased if you could give me a hand on...

24 January 2021 5,984 4 View

How do you control the cancer?

25 October 2018 7,240 3 View

Is there a role for empirical oral steroids in children with severe behavioural disturbance following acute viral encephalitis?

Post encephalitic behavioural disturbances is one of the poorly researched area with regards to patient management. Role of immunomodulatory therapy in post viral autoimmune encephalitis is well...

09 October 2018 3,344 0 View

What are other ways of increasing the effectivity of RT-PCR in testing for the Japanese Encephalitis Virus in mosquitoes?

I have read about RT-PCR as a molecular amplification assay for detecting the presence of flaviviruses. However, when used on the extracted RNA of mosquitoes carrying Japanese Encephalitis Virus,...

19 April 2017 3,104 3 View

HSV encephalitis: Does anyone have experience with a CSF test for HSV PCR after three weeks treatment with acyclovir?

We encountered a serious case with HSV positive encephalitis. After three weeks treatment with Acyclovir, we did again a lumbar punction. The PCR turned out to be still positive. Is there anyone...

04 April 2017 4,240 2 View

HSV encephalitis: Does anyone have experience with a CSF test for HSV PCR after three weeks treatment with acyclovir?

We encountered a serious case with HSV positive encephalitis. After three weeks treatment with Acyclovir, we did again a lumbar punction. The PCR turned out to be still positive. Is there anyone...

03 April 2017 8,507 2 View

Tick Borne Encephalitis Virus NS1 antibody for WB and IF?

04 May 2016 4,197 2 View

There are three state for N classical particles with energy 0,kT,2kT. the equlibrium energy of the systemis 1000kT,what is the value of N?

statical mechanics

02 March 2016 7,634 1 View

Is it possible to isolate/detect Japanese Encephalitis virus from Bird droppings?

Migratory birds are likely to transport JE viruses from an endemic area to a non-endemic area. Screening their blood would be the best method to detect JE virus in them. However, it is not...

03 April 2015 9,876 13 View

Is it possible to isolate/detect Japanese Encephalitis virus from Bird droppings?

Migratory birds are likely to transport JE viruses from an endemic area to a non-endemic area. Screening their blood would be the best method to detect JE virus in them. However, it is not...

01 April 2015 8,156 12 View