Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

How can I convert .vesta file to a .cif file?

I've used the "export data" option in Vesta software to convert my unit cell into a cif file however when I use that cif in atomsk it shows error such as "can't read the position of atom 0". If I...

28 February 2021 4,966 3 View

What are the things you should keep in mind while designing a controller for a quadrotor and want to implement it on hardware (via arducopter) ?

I am designing a flight controller for a quadrotor. At first I am designing a nested/cascaded controller consisting of only proportional controllers Kp . Now, if i tune the rate controller for 10...

03 February 2021 5,001 3 View

How to discretize a continuous time state-space model in MATLAB correctly?

I am trying to discretize a continuous time state space model using the following code s=tf('s'); G=1/(Iyy*(s^2)) Gs=ss(G) Gd=c2d(Gs,0.01,'zoh'); Now, when i use this discretized model 'Discrete...

30 January 2021 8,665 10 View

If i want to use annual time series data, but the number of observations are less then requirement, so i can convert data in quarters?

If i want to use annual time series data, but the number of observations are less then requirement, so i can convert data in quarters?

27 December 2020 7,304 19 View

Can I use conventional PCR master mix instead of hot star Taq polymerase in DNA methylation studies or in bisulphite converted DNA ?

I am trying to predict age from DNA methylation. I am using conventional PCR master mix, but I didn't get my PCR product. Is it due to Master Mix? Should I use HotStar Taq Master Mix ?

08 December 2020 8,776 4 View

How can I download Materials Studio ?

How can I download Materials Studio ? Anyone please suggest me any website. Thank you

10 November 2020 5,238 3 View

Is there a way to implement Kalman filter on three dimensional GPS data to remove noisy data?

I am trying to implement Kalman filter to get rid of multipath error points on GPS data while my data contains Latitude, Longitude, accuracy, altitude, speed and time. I am using python but unable...

01 November 2020 8,837 3 View

A sample size near to 20 with pre and post assessment after psychotherapy sessions. Can we apply paired sample T test? or other suggested tests?

A sample size near to 20 with pre and post assessment after psychotherapy sessions. Can we apply paired sample T test? or other suggested tests that we can apply on it?

15 October 2020 6,339 5 View

How to choose output capacitor for converters connected to a common dc bus of a SST based dc microgrid?

I am trying to integrate the low voltage dc bus of a solid state transformer(SST) with a dc microgrid. My SST and DC microgrid are operating fine individually. But when i try to connect all of...

11 October 2020 2,090 1 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View