I have a plant extract and I want to know its effect on humans and what is the lethal dose LD50 that I should recommend
Hope that the file I gave the link may be a support for you in this regard. You can also surf internet to know the LD50 doses for different botanical such as Pyrethrum, Azadirachtin, Nicotine etc. And use those dose as pilot test. http://www.mdpi.com/2072-6651/9/3/75/pdf
What type of plant extract? you need to do preliminary experiment on 3 mice using enough literature about the dose found to be safe for mice.
Best regards
Aly R Abdel-Moemin
Dr Aly R Abdel-Moemin thank you for your answer but there is no literature about the dose found about my plant ex.
Try several doses on a small scale of mice and follow them for a week
I suggest you use dose conversion according to Laurence dan Bacharach (1964)
Hope that the file I gave the link may be a support for you in this regard. You can also surf internet to know the LD50 doses for different botanical such as Pyrethrum, Azadirachtin, Nicotine etc. And use those dose as pilot test. http://www.mdpi.com/2072-6651/9/3/75/pdf
Thank you very much Md Sahadat Hossain this academic research is very useful to me
I think the Lorke 1983 method is the best method to estimate LD50 for a plant extract, which is based on the treatment of three groups of mice in doses of 10, 100, 1000 mg / kg and from the resulting death results. Another trial is conducted on 4 other mice in doses up to 5000 mg / Kg.
Dear Ammar K. Jasman,
usually dose finding study is conducted as per OECD guideline 423 which is recent one, requires very few number of animals and accurate. (OECD GUIDELINE FOR TESTING OF CHEMICALS: Acute Oral Toxicity – Acute Toxic Class Method, Adopted: 17th December 2001).
kindly find the attachment, go through the flow chart given in the OECD Guideline 423.
at the end of this study you will get LD50 cut-off value.
one tenth of LD50 is ED5O.
then you can fix three doses.
LD50 value of most of the plant extract would be around 2000 to 5000 mg/k.g body weight.
for example,
LD50 is 2000mg/k.g body weight
then ED50 would be 200mg/k.g body weight.
lower, medium and higher doses are
T1-200, T2-300 and T3-400 mg/k.g body weight respectively. or two groups
T1-200 and T2-400.
three groups would be appreciable with positive control and disease control.
if you have any further doubt, mail me. I will try to clear it.