for stimulate jurkat cells for cytokine produce with PMA/ionomycin/ brefeldina A how much brefeldin A should i use for stimulate million jurkat cells with 1-2 microgram/ml calcium ionophore and 10-20 ng/ml PMA?
Charles Chang
BFA is used to prevent secretion of proteins, not to stimulate cells.
Working concentration is 10ug/ml
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