How much brefeldin A should i use for stimulate million jurkat cells with 1-2 microgram/ml calcium ionophore and 10-20 ng/ml PMA?

for stimulate jurkat cells for cytokine produce with PMA/ionomycin/ brefeldina A how much brefeldin A should i use for stimulate million jurkat cells with 1-2 microgram/ml calcium ionophore and...

08 August 2016 4,453 2 View