How to do MD simulation of docked protein complex after docking .?

I have perform some protein-protein docking studies. I used Hdock, Prizm. But I am unable to find a proper method to verify my docked structure. I also docked my structure in Hex program to get...

19 October 2020 4,083 7 View

How can we measure the content of happiness in a person by applying various mathematical and soft computing techniques?

Here I just want to know about the actual parameters to measure the content of happiness in a person. With the help of these parameters a neural network can be generated and maintained to achieve...

22 September 2020 7,666 2 View

Which tool to use for Sample Size Calculation studies for AI based studies involving Machine learning or Deep Learning?

I want to know of an online tool or formula to give a vague idea about minimum number of enrolees for a study involving use of machine or deep learning.

01 August 2020 747 3 View

What all we can do to troubleshoot a genotyping PCR?

We have been characterizing our transgenic mice lines as a routine job in lab for years and It has been working fine until a recent batch of primers for VEGF has arrived. We started observing...

28 July 2020 7,639 15 View

What could be the potential reasons for a long DNA molecule not able to attach to the mica surface for AFM imaging even in the presence of Mg++ ?

29 June 2020 2,075 2 View

How Machine Learning Algorithms can be applied for the High Altitude Wetland restoration? Any idea how ML can be integrated with Geospatial Tools??

Wetland Extent Mapping

09 February 2020 3,921 3 View

How to find gene with similar function across Bacteroides and Firmicutes?

My genes interest is EntA in E.coli. I want to find genes with similar function like EntA in any members of Bacteroides and Firmicutes. I need the DNA sequence of EntA-like genes in these phyla.

01 January 2020 2,400 1 View

I am looking for the melting point and heat of fusion of graphene oxide. Could someone please help me in this regard?

Any reference in this regard would be very helpful.

06 December 2018 6,561 3 View

Biofilm Fixation for Confocal?

I have grown biofilm and I need to fix it and stain it. 1. Clean the entire disk carefully and aseptically with pipette so that extra biofilm which are present over the walls. 2. Cells are fixed...

17 July 2018 3,954 5 View

What would be the best for miRNA NGS validation?

I would like to validate miRNA NGS data by qRT-PCR. This theoretically could be done from total RNA samples (the one used for sequencing purpose).What approach should I use to isolate the miRNA or...

25 February 2018 4,574 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

Color of spiro-ometad film?

What does the color of a spiro-ometad film indicate? I got purple, green, red, even black color of the spiro film. Is there anything we can get from the preliminary color analysis(e.g. oxidation...

21 February 2021 1,831 4 View

How to successfully revive cells stored at -80 degree C for a year?

I am working on C6 cells along with other neuronal cell lines. We have preserved the cells in 90%FBS and 10% DMSO solution. We are reviving the cells after a year and cells are either not binding...

18 February 2021 4,499 1 View

What are alternatives for B27+ Supplement in neuronal cell culture?

Hello everybody In our iPSC-derived neuronal culture we use Maturationmedium, supplemented with B27+. As Thermo Fisher run out of stock and can deliver earliest in May, I wanted to ask whether...

16 February 2021 3,348 1 View

What statistical test is required for an analysis on pre-COVID & post-COVID patient improvement after attending a 10-week programme?

Hi all, Myself and my supervisor are looking at carrying out a statistical analysis to see whether there is a difference in patient outcomes for our 10-week pain management programme according to...

10 February 2021 6,622 10 View

RWP1 growing in RPMI medium?

Hello everyone, Has anyone try to culture the normal prostate RWPE-1 cell line in RPMI medium supplemented with foetal bovine serum? There's some articles available but neither describe details...

08 February 2021 6,455 1 View

Should GFP sequence be cut together with the IRES sequence?

Hello. I have a viral plasmid which contains a bicistronic region: CMVprmtr-GFP-IRES-PuroR. I would like to cut out the GFP sequence to get rid of the green fluorescence. In this case, would it be...

02 February 2021 1,644 7 View

What is the most appropriate statistical method for comparing presence/absence data (plant ecology)?

I have a dataset made by plant species as presence/absence (1/0) found in 133 samples within an archeological area. Every sample has a particular substrate, a position in the monument and it is...

21 January 2021 3,585 7 View

Does anyone know the pH-differential method?

I use the water method to extract the anthocyanidin from Peristrophe bivalvis (L.) Merr leave, then dilute 20 times with 0.025M potassium chloride, pH1.0 and 0.4M sodium acetate buffer, pH4.5...

20 January 2021 8,305 4 View

How to remove colour from DNA pellet during DNA extraction?

I am working with Amaranth accessions which are green to red in color and contain high phenolic compounds. Using the CTAB method with the slight modification I am trying to get high-quality DNA so...

18 January 2021 3,578 1 View