When I try to collect the earthworm specimens in each spot, I found it's really hard to get enough objective samples in some plots, maybe 1 or 2 or 3 specimens in a plot. However, limited specimens mean pretty limited genetic variation, and actually, the number of samples has a strong relationship with genetic diversity.
Could somebody tell me something about your experience?
Csaba Csuzdi
I think there is not an "absolute number" however in a simulation it was shown that after 20 specimen heterozygosity does not change much. (doi:10.1371/journal.pone.0045170).
Perhaps it is true for earthworms as well.
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Partha Sarathi Tripathy
Large sample size, especially 20% of the population of earthworm you are selecting for better results statstically...
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