My question is how many times is it worth reprocessing a MGIT contaminated with bacteria? I presume this would depend upon whether AFBs are seen or not? I can't find any advice in national SOPs or the manufacturer handbook. Many thanks Andrew
Hello, So I have mouse brain tissue MS data from one of my predecessors and I have human serum data from the hospital that I collaborate with. We are trying to find markers for the diagnosis of...
11 July 2024 8,484 0 View
Mine is sitting here: http://openkan.org/DLpiecewiseCPP.html I wish to see someone else's.
06 July 2024 9,974 1 View
I'd trying the several countries with several religions on young and older. what are which books so far. Sound Medicine, Music Medicine on many religions working now.
18 June 2024 1,907 1 View
I have read a few papers that have indicated that the sampling rate of the Polar h10 is 1000 Hz. 1) Navalta et al., Heart rate processing algorithms and exercise duration on reliability and...
10 June 2024 4,744 1 View
I have already tried different combinations of optimized DNA Nicking Assay protocols. The plasmid DNA that I use is puc19 from a transformed E.coli. Every single time I perform my Assay, I always...
30 May 2024 1,457 2 View
Hello everyone, I would like to culture muscle cells, specially Human Umbilical Aortic Smooth Muscle Cells (HUASMCs) in DMEM. There are various papers out there where this has been done, however,...
28 May 2024 1,398 0 View
wetland degradation; over exploitation of wetland lands /swamps. Drivers; causes/factors leading to
25 May 2024 3,382 1 View
I've used the basic formula : 95% confidence interval = sensitivity +/− 1.96 (SE sensitivity) Where SE sensitivity = square root [sensitivity – (1-sensitivity)]/n sensitivity) 95% confidence...
21 May 2024 2,227 1 View
Hi all, I have a question regarding something odd I'm seeing with DAPI on tissue. I'm trying to isolate the issue to prevent it in the future. I have sagittal brain sections on slides. For some...
15 May 2024 9,194 2 View
As the title states, I am looking for a housekeeping protein for serum (for Western blots). This research is in the context of pregnancy so it has to take into account normal vs diseased (e.g....
15 May 2024 9,219 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
Hi, Now I am doing a single-base editing by using HiFi Cas 9, guideRNA, single stranded DNA, HDR Enhancer V2 and my tranfection mehtod is lipofection (RNAiMax). Unfortunately, after gene editing,...
26 July 2024 2,136 0 View
I suspect my cells are contaminated with mycoplasma. I fixed the cells with 4% PFA and stained them with DAPI. Below is the image I obtained. I don't observe the typical small, rounded DAPI foci...
11 July 2024 7,786 3 View
There are black dots that seem like contamination in my corneal endothelial cell cultures. Some of the cells appear unhealthy and are enlarged. The black dots are more prevalent around these...
11 July 2024 4,829 0 View
The question refer to leafy biomass decomposition using litterbag techniques.
11 July 2024 2,078 0 View
When Streptomyces undergoes conjugation transfer, it is always contaminated by a type of yellow Pseudomonas. What could be the reason for this?
07 July 2024 5,480 2 View
What is meant by cross-contamination and what causes fruits and vegetables to become contaminated during preparation and storage?
02 July 2024 9,204 0 View
We have been experiencing repeated what looks very much like fungal contaminations in several incubators (in the same lab). We have tried the following repeated times and yet the coating on all...
02 July 2024 6,375 2 View
Within our research lab we have access to PFOA in powder form and PFOS in 100microgram/L methanol solution. We want to make a PFAS contaminated water to test a PFAS treatment technology. Does...
01 July 2024 8,425 3 View