I already used the DEAE cellulose column once for separating compound, how many times we can reuse the column with proper washing?
I used 1 M NaCl as the elution solvents so, which solvent is better for washing the column for better use?
1. “Resting Heart Rate, Heart Rate Variability and Electrocardiogram Changes In Chronic Alcoholics” was published in journal of medical sciences and clinical research volume 03 issue 02 February...
26 July 2023 1,172 3 View
Hi, could anyone suggest which statistical analysis is used to analyze the data of pretest-posttest control group design with two dependent variables and one independent variable? your explanation...
09 September 2021 2,862 7 View
As many studies identified of genetic variants linked to training responses and sport-related traits, we could hypothesize that the R allele was more common in sprint and power athletes and the X...
19 February 2020 3,477 3 View
Hi, I'm a management student that want some help for finding the excel data of Economic Risk Rating from 2014 to 2019 or at least to 2018. If there's anyone who can help me please share the data...
13 January 2020 1,286 1 View
Hi all, I did PolyNIPAM-Cellulose grafting process using Ceric Ammonium Nitrate and HNO3 as catalyst. I did the experiment for many times. It can be concluded from 10 experiments: - 3 experiments...
20 February 2019 3,260 3 View
I just started do research about polyNIPAAM last month. I always wear complete laboratory equipment (laboratory coat, gloves, and mask) in every experiment, the laboratory is also equipped with...
11 July 2016 713 2 View
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We stain our fibroblast culture (designated for stem cell feeder cells) with AP, the results show a few red stained cells? is this a AP+ results or a false positive? is this means that there is a...
28 December 2015 6,494 6 View
I'm doing a research about nanocellulose, I hope I can get porous film from nanocellulose by simple drying method, but it can't. The nanocellulose solution will form continuous film after drying...
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fresh foreskin, short decontamination procedure using povidone iodine and EtOh, overnight 4 C incubation in dispase II 1 ug/ml diluted in PBS w/ Ca, Mg for dermal-epidemal separation, epidermal...
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A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Evolutionary fitness is based on an organism’s ability to adapt rapidly to changing environmental circumstances. Large-bodied mammals have been equipped with large brains (and hence a high...
06 August 2024 4,849 2 View
Are there any suggestions or insights you can provide to help address this problem
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Air moisture harvesting Air water collection devices
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I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View