Do you mean to use AAO as a master for soft lithography, PDMS stamp production?
If that's the case you first need to evaporate anti-sticking surface coating (silanization). And then you can use the master couple of times (min 10 times) for templating. But it will gradually become sticky and you will be able to see it, giving you an idea of how many more times you can use it. To my experience the average is 30 times.