Where is the error in the computation of coefficients of Baker-Campbell-Hausdorff formula?

I have a Lie Algebra with a basis: A_1 = [[0 1 0], [0 0 0], [-1 0 0]] A_2 = [[0 0 1], [-1 0 0], [0 0 0]] A_3 = [[0 0 0], [0 1 0], [0 0 -1]] The Lie brackets are [A_1,A_2] = A_3 ; [A_1,A_3] = A_1...

21 October 2020 1,479 6 View

Looking for the legacy Opticstudio Zemax version 15?

Can someone provide the installation file Zemax Opticstudio15? We have the USB key with the license but not anymore the installation file and Zemax does not provide legacy versions older than...

16 January 2020 588 10 View

How to interpret regression results if dependent variable is logged and independent variables are standardized?

In my fixed effects panel regression analysis the DV (Triadic Patent Families) enters in logarithmic form. To counteract very small coefficients because of different scales in my IVs and enable...

17 December 2018 3,020 1 View

Is Carboxymethylcellulose stable over few day ?

Does anyone knows if carboxymethylcellulose is stable during 10 days. We should provide a drug to our animals and we will use CMC as a vehicle but the dose of drug avec very low so I'm wondering...

28 February 2018 8,546 3 View

In pop culture, do we remember best the artworks that create a sense of unanimity or those that elicit debate?

This question is motivated by the recent debates that sparked around the release of Star Wars: the Last Jedi. The work is challenging the philosophical assumptions of many fans and is eliciting...

22 December 2017 5,677 3 View

I'm planning a glucose uptake assay.

I'm planning a glucose uptake assay with adult mesenchymal stem cells and possibly rat neural stem cells for my undergraduate thesis. i'd appreciate some recommendations for uptake kits and...

18 July 2017 2,222 1 View

Does anybody have primers for human/mice IFN Alpha and Beta?

Hi everybody, For my Master Thesis I am working on Orthobunya Viruses and for infection studies (in mice, Huh7 cells, etc.) we want to meassure IFN alpha and beta expression, as well as the...

22 March 2017 2,691 2 View

Who did the best mechanical shear-flow experiments for shale ?

At low strain with shear loading and unloading steps.

28 May 2016 344 3 View

Are proteins bound to the extracellular matrix extracted upon lysing cells by scraping in lysis buffer in a PBS-washed petri dish ?

I am wondering if extracellular protein are also taken out when we extract protein directly from a dish (PBS-washed cells). Many protocols appear to detach cells with EDTA and then extensively...

10 May 2016 1,556 2 View

Are there Quantum sensors to measure cosmo-telluric energy ?

is there any interesting quantum sensor to measure cosmo-telluric energy ?

17 November 2015 7,711 29 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View