How many plants must be sampled in each group (if 2 groups are present) for constituting the bulk for a Bulk Segregant Analysis (BSA)?

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Mostafa Haghpanah

hi dear Shahid Iqbal Awan

The number of sample in BSA study completely depended to the trait.

This link can be useful for you.

best regards

http://onlinelibrary.wiley.com/doi/10.1111/pbi.12559/full

Kamal Kumar

I agree with above suggestion.. It also depends upon distribution of analysed trait in RILs..if extreme ends in the trait distribution graph have a highly reproducible data with 15-30 different RILs then it is good to go with QTLseq like technique..

Shahid Iqbal Awan

Thanks Mr. Haghpanah and Mr. Kumar for response