Ta (73) is an ideal target material for accelerated proton beams
I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...
22 February 2021 10,099 5 View
Hello, I am planning to insert GOI between CaMV 35 promoter and mGFP in pCambia1302 to produce a fusion protein and see subcellular localization of the protein. But the vector says it's membrane...
15 February 2021 4,353 3 View
Hello. I am currently trying to express a recombinant gene with Avitag at the N-terminal. I'm aiming at a final product that looks like this: Avitag-(target protein) The protein I use is already...
14 February 2021 2,039 4 View
My colleague and I are having some trouble mounting 70 micrometer mouse brain slices. When we initially position the coverslip over the mounted slices, there are no bubbles but when we come back...
10 February 2021 3,580 1 View
I am looking into DNA methylation of mice as an aging marker along with other assays and recently I added brain IF to the list of assays. For this, I need to fix the brain by transcardial...
09 February 2021 9,012 3 View
I am trying to visualize alpha and dense granules in platelets. However, it seems like the granules were not stained well. Is there any way I can improve my staining technique to obtain better...
04 February 2021 5,925 9 View
I synthesized 1T/2H hybrid phase MoS2 on a graphite foil surface by hydrothermal method, and then analyzed it by Raman spectroscopy. As a result, three new peaks correspond to the 1T phase were...
04 February 2021 6,580 2 View
I want to simulate a uniform stress condition in the vertical direction on the surface of a spherical particle. Since it is very difficult to describe the boundary conditions of a complete...
31 January 2021 2,726 1 View
Surely they will want to avoid contact with air/moisture, so what is the procedure for this?
23 January 2021 428 2 View
I have a lack of knowledge on sample preparation for RNA extraction to test molecular diagnosis. I just want to know whether it is plausible to mix TCEP-HCl lysis/reducing buffer and NaI...
21 January 2021 9,309 1 View
I am trying to simulate a simplified version of a beam resting on 10 points using Abaqus. The solutions I want to obtain are the Reaction Forces in each resting point or area when applying a...
21 February 2021 3,075 2 View
I am trying to simulate the impact of a sinusoidal force on a Beam, yet I registred this beating signal (Displacement by time). The freaquency of excitation was the same as the first natural...
19 February 2021 5,872 3 View
Hi, If I am performing an MD Simulation in which the two groups/molecules are moving in a direction on applying a force on the system, how would I calculate the velocities/acceleration of the two?...
08 February 2021 9,525 4 View
Hello, I'm performing system identification of the lateral closed-loop dynamics of a quadrotor. My model receives a setpoint and should return position and acceleration. I've proposed a second...
07 February 2021 9,791 2 View
I have some basic questions about the behavior of a charged particle in a static B-field. 1. Without a B-field, the particle has spin, with random orientation and spin frequency. a) What...
24 January 2021 1,389 3 View
I wanna to have proton energy according to their number ( E=f(N) ).
23 January 2021 2,788 2 View
Hi, I need to do analysis on cantilever beam of piezoelectric material. Apply force or excitation on free end and want to find out voltage. I want to do it in ansys work bench. Please let me...
21 January 2021 2,134 3 View
I try to pattern the gold layer around 2 nm-20nm on silicon, I used around 2000nm thickness (PMMA 950K A13), I tried to pattern five samples in total, but only one showed the pattern I wanted. I...
13 January 2021 7,710 1 View
Am in Peptide / Protein Research, may i know what useful analytical information we are getting from 13C & 1H NMR spectroscopy analysis? Somebody getting NMR for smaller proteins, any useful...
05 January 2021 1,196 7 View
Why does normal bending stress/flexural stress value (Sx) just direct below a concentrated load of simply supported beam which is compression stress higher than the hand calculation Exact solution...
03 January 2021 8,462 3 View
