How can we detect over expression of a gene?

Sometimes some gene gets over expressed in organism, so which molecular technique can help us to detect the over expression of that gene?

06 July 2019 9,406 5 View

How many bands will I find if I run human extracted DNA through agarose gel electrophoresis?

Human bears separate 46 chromosomes. If we extract DNA from human sample then how many band will be visible on gel?

08 September 2018 6,350 5 View

Do pH maintaining chemicals interfere in DNA extraction?

We use EDTA, Tris-HCl and some other chemicals, whose pH remain almost 8.0. To balance pH 8.0 we use NaOH. Now I would like to know that; have these unwanted NaOH, HCl or Acetic Acid (if...

04 May 2018 5,684 1 View

Which is the solution used for pH meter sensor?

I need to know that which solution is used to keep the pH meter sensor wet. Some people say that KCl is used but which concentration and pH are better?

31 December 2017 5,670 3 View

How can we measure the band size that greater than 10Kb?

07 August 2017 3,413 2 View

Why do bacteria lose their plamids at normal condition?

Plasmids are extra chromosomal DNA of bacteria, but they have numerous functions for the survival and living of bacteria. It is claimed that bacteria lose their plasmids when they live without...

07 August 2017 3,918 9 View

Is it mandatory to culture Bacteria in an antibiotic rich medium before plasmid extraction?

It is claimed that bacteria lose their plasmids unless they are cultured in a antibiotic maintained medium. As a result a negative consequence will arise after plasmid DNA extraction. How will you...

07 August 2017 1,637 6 View

Where are the nod and nif genes of Rhizobia located?

07 August 2017 9,745 2 View

Is it possible to culture coffee plant in vitro?

Is anywhere it being tissue culture of Coffee plant in Bangladesh?

04 May 2017 4,613 2 View

What is selfish Rhizobia?

Somebody claims that there some selfish rhizobia which take benefits from the host but do not induced nodulation at the host. I did not get enough evidence about this. Could anybody help me by...

07 August 2016 3,089 6 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View

How relevant is Mini slump cone?

for all the rheology researchers, my question is, how relevant is a mini slump cone (smaller than a standard 50 cent rheometer). currently based on testing, the thickness of slumped material is...

26 February 2021 3,668 1 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View