How many cycles are good for shoot tip culture of banana?

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Can we mark 'EFL Learners shifting from general digital to AI technologies' as technological transition?

After COVID-19 it has seen that EFL learners technological affiliation has raised. In addition, in the post-COVID period learners started to engage AI technologies like ChatGPT while learning...

08 August 2024 8,964 4 View

How to generate a citation of my paper from ResearchGate?

How we can cite the papers from ResearchGate. I am trying to create citations for this article, Quantum Machine Learning Algorithms for Optimization Problems: Theory, Implementation, and...

08 August 2024 6,690 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Why does everyone use vs code?

Visual Studio Code (VS Code) has become a popular choice among developers for several reasons: 1. **Free and Open Source**: VS Code is free to use and open source, making it accessible to...

07 August 2024 7,013 4 View

How to fix background error in rietveld refinement of one XRD peak using GSAS-II?

I want to refine one XRD peak of my in-situ xrd but the background is never working good which ultimately fails the refinement. How to refine and adjust the background using GSAS-II

05 August 2024 5,291 2 View

How can I add own Henry coefficients in Aspen Plus?

Hi, i would like to simulate an absorption process in Aspen Plus. I want to use the NRTL model und would like to add some individual Henry coefficients. Is that possible and how?

05 August 2024 2,333 2 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

Can usage of AI tools like chat GPT in research work is recommendable ?

AI tools like ChatGPT can enhance research work significantly when used responsibly and in conjunction with thorough human oversight.

05 August 2024 1,842 3 View

Usage of internal standards in LC-MS/MS analysis?

Have you ever seen a LC-MS/MS method uses both internal standards and external standards (in matrix matching purpose) but the concentrations of internal standards are outside the calibration curve...

05 August 2024 3,084 6 View

ANY free software for reconstructing neurons in the microscopic image?

Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...

04 August 2024 4,725 2 View

If my gene of interest has high GC content can it be problematic in sequencing? What kind of error is expected with GC rich gene sequences??

Gene sequencing related trouble shooting

25 July 2024 4,149 2 View

Is it possible that western blot result to be like this?

Hai, Iam doing experiment about novel compound to induce thermogenesis on 3t3-L1 cells. The beta actin has been adjusted, but when i did western blot and imaging with image J, the result it is...

14 July 2024 3,354 1 View

How does the foilar application of Banana Pseudostem sap influences the growth as well as yield attributes of oilseed crops?

Also, explain its pros and cons concerning other organic sources of nutrients.

01 July 2024 612 0 View

Can I premix the reagent for restriction digestion and make a mastermix like PCR?

Hi everyone, I am currently trouble shooting my restriction digestion, so far it hasnt given me clear bands when I ran gel. I've been adding all reagents minus template, to make a master mix...

24 June 2024 1,171 3 View

After plating mouse primary neonatal cardiomyocyte we do not observe beating after a few days, any suggestions as to how we can trouble shoot?

We coat the plate in poly-D-lysine (50 ug/mL) followed by fibronectin (50 ug/mL) before plating the cardiomyocytes. After isolating and plating cardiomyocytes from 2-4 day old pups, we fed the...

20 June 2024 393 0 View

Shooting Method in Matlab?

can any one provide shooting method in matlab for 9th order differential equations with 9 boundry condition(Velocity, Temperature and concentration)

14 June 2024 7,928 2 View

Example table for this thesis “an analysis of particle board from banana pseudostem, EPS waste and sawdust using Taguchi method”?

test on particle board by density, water swerlling and tensile strength. Just need example amount how much we use in this experiment

29 May 2024 5,366 0 View

What are the extraction methods that can be used to extract formaldehyde component of a fruit e g banana/orange?

AAZ

11 May 2024 7,466 2 View

I am working on stress plant physiology. How can i calculate the root length, shoot length of plants in percentage(%)? Any specific formula??

I searched in literatures and i got the formula root length of treated plant / original root length *100 when i applied this, i got the values higher than 100.

10 May 2024 9,148 2 View

What is the public health impact of firearms conversion devices?

I am reaching out regarding the issue of conversion devices/auto sears/switches that make it possible to convert semi automatic pistols and rifles into full automatic. These devices are...

19 April 2024 8,796 0 View