I am planning to isolate MAM from an epithelial cell line.What is a good amount of cells to use? I found a large variety of suggestions, from 5 dishes (10cm diameter) to 80. What do you suggest?
I have a question about MAM isolation. Always when I'm resuspend the crude mitochondria I broke the OMM and the nature paper suggested to ressuspend with paintbrush or with the pippete, but cut the end of the tipp. I'm doing that and didnt work out. I would like some suggestions.
just wondering whether you were finally able to solve the issue on MAM isolation? I would appreciate it if you could please share your option for resuspending the crude mitochondria.