Hi everyone. Maybe you have any references on the ranges (low, moderate, high) of nucleotide diversity to support on my manuscript writing. Your response is highly appreciated. Thanks!
Nucleotide diversity refers to the relative proportion of nucleotides A, C, G and T present in every cycle of the run. Well-balanced or high diversity libraries have roughly equal proportions of all four nucleotides in each cycle throughout the sequencing run. Low diversity libraries have a high proportion of certain nucleotides and a low proportion of other nucleotides in a cycle. Finally, libraries that have in-line barcodes or other molecular identifiers can have regions of low diversity in otherwise high diversity libraries.