How long would you recommend for human Th polarization ?

10 May 2021 1 9K Report

Hi,

I am polarizing human naïve T CD4+ cells into Th1, Th2 and Th17. How long would you recommend for each culture ? Is it possible to synchronize the three ? We want to analyse their phenotype by flow, using RORgt, T-bet and GATA3.

Thank you !

Peter McClintock

Sorry - I do not know! Peter.

More Joëlle Plourde's questions See All

What M1 and M2 markers can I use for flow cytometry ?

Hi, I'm a student and I'm doing an internship. I need to asses the M1 and M2 populations of a culture using flow cytometry. From my researches, I know that this is subject to some controversy, as...

26 January 2021 299 3 View

For PC-3 cell line. How long before irradiation do I have to synchronize my cells to G0/G1?

I am trying to do a clonogenic assay for PC-3 after irradiation. I have to synchronize my cells by serum starvation but there's a optimal time I have to do that before irradiation. been looking in...

09 February 2021 9,480 1 View

Grid connected inverter using virtual synchronous machine?

Hi everybody, currently, I am working on the grid-connected inverter. I want to make my grid-connected PV system a PLL-less system. I have studied some reachers are implementing a virtual...

07 February 2021 6,078 2 View

Can you polarize Jurkat cells into different T helper subsets?

I am wondering if anyone has had success with in vitro polarization (via cytokines) of Jurkat cells. My goal is to understand the affect of a drug on Treg induction and differentiation. I...

12 January 2021 3,606 2 View

What is the effect of polarization of unit elements in an IRS, if its built for mmWave application?

I usually don't see path loss models for IRS consider polarization. But specific to mmWave application, the polarization of the antenna has some effect. Is there any research that has explored this?

08 January 2021 3,054 1 View

How to choose mouse cell line to work on?

Hey, I am looking to start working on mouse cell line for some genomics experiments. I have the following requirements: 1) Normal chromosomes composition. 2) Possible to transfect. 3) Possible to...

03 January 2021 4,387 2 View

Why is emotiv epoch+ contact quality 0% ?

I am trying to use new emotiv epoch+ headset which was bought in 2018. I am having half of the electrode as green Like this picture. But my question is why the over all contact quality is 0%...

17 December 2020 9,614 2 View

How to drive the free electrons present in ferroelectrics when saturation polarization occurs?

In the applications of external electric energy to ferroelectric materials, the internal polarization drives the free electrons up to saturation polarization. So after saturation polarization,...

15 December 2020 832 4 View

Does increasing the number of individuals by copy-paste of the same indvs to perform GWAS affect the results?

I am trying to perform a genome-wide association on mitogenome variants (obtained from GATK-Mutect2) vs. phenotypes by using GAPIT. The number of individuals is seven and GAPIT function does not...

14 December 2020 8,853 3 View

Question on calculating the static mean polarizabilities using Gaussian Output files?

I performed an opt calculation on molecule A and got A_opt. I performed a freq calculation on A_opt and got A_opt_freq I performed a polar calculation on A_opt and got A_opt_polar. The A_opt_freq...

09 December 2020 1,188 1 View

How long do mouse CD4+ T-cells remain activated after removal of CD3/28?

Hello, I would like to analyse naive CD4+ T-cell activation and intracellular processes with a machine that does not allow CD3-coated plates. I thought of activating my cells with plate-bound...

08 December 2020 3,536 1 View