Joëlle Plourde

4 Questions 3 Answers 0 Followers

Questions related from Joëlle Plourde

Has anyone ever managed a good M2 polarization from Thp-1 or HL60 cell lines ?

Hi, I am really at a loss here. I am differentiating Thp-1 into macrophages, and then polarizing them into M1 and M2 macrophages. My M1 profile is basically flawless, but I can't get M2s for the...

06 December 2022 1,452 0 View

QPCR : Is it okay if a gene is more expressed than the housekeeping gene ?

Hi, I am working with a Thp-1 cell line and doing polarization towards M1 and M2 for further experiments. To confirm I did end up with the right profiles, I am doing qPCR on the undifferentiated...

29 November 2022 5,247 5 View

How long would you recommend for human Th polarization ?

Hi, I am polarizing human naïve T CD4+ cells into Th1, Th2 and Th17. How long would you recommend for each culture ? Is it possible to synchronize the three ? We want to analyse their phenotype...

10 May 2021 8,618 1 View

What M1 and M2 markers can I use for flow cytometry ?

Hi, I'm a student and I'm doing an internship. I need to asses the M1 and M2 populations of a culture using flow cytometry. From my researches, I know that this is subject to some controversy, as...

27 January 2021 476 3 View