how long fungus mycelia could be preserved for TBARS ( thiobarbituric acid) assay. as i have Ganoderma lucidum mycelia preserved since 8 months and i am not sure whether or not it is still ok to measure tbars in?
why TBARS (Its a synthetic antioxidants) ok its a strong antioxidant but not healthy, i suggest vitamin C, natural dried extracts
The most important factor for preservation is temperature, since unsaturated fatty acids are unstable even if at - 60⁰ C. If your samples were preserved at - 80⁰ C, you can use them for TBARS analysis.
Most of the test fungi could survive for 5 years in medium containing 10% glycerol even after alternate freezing and thawing at intervals of 6 months.
https://www.sciencedirect.com/science/article/pii/S134035400270675X
Kindly check
https://scialert.net/fulltext/amp.php?doi=ijbc.2015.79.85
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