Also, is it possible to speed it the process? And what's the best protocol to use. Based on my current protocol, it should take 14 days/ 2 weeks + maintenance = 3 weeks total.
With practical examples/illustrations
22 July 2023 9,121 3 View
1. May I do a paired-end 150 bp (2x150bp) sequencing for the ATAC-seq library? Or do you recommend other read lengths? 2. Please correct me if I'm wrong. If I am using 2x150bp, these 2x150 bp...
23 May 2021 9,687 2 View
Hello everyone, Please I will like to get your ideas on how to calculate the d-band center of a bimetallic crystal. My intention is to eventually see how the d-band center affects the performance...
21 April 2021 7,150 2 View
I am unable to achieve convergence in my VC Relax calculation for a doped Bismuth structure using Quantum Espresso. When I ran the calculation for only Bismuth it converged but after I doped it...
05 April 2021 5,492 5 View
I intend to calculate the transition state energies for methane pyrolysis reaction over a transition metal (catalyst) using Quantum Espresso for the DFT calculations. I am looking for any...
05 April 2021 552 1 View
The debate about the death penalty have been one that has been around for years, it is something that has always interests me . For my major research project to finalise my degree as a student, I...
04 October 2020 6,955 9 View
hello all, I'm current doing a project in my final year of university based on anxiety affecting those with dyslexia and dyspraxia and would like to hear what your opinions on it or any research...
30 September 2020 8,775 6 View
hi i am a student doing research on stress in teachers and their coping mechanism if any one can answer this question it will be much appreciated or even point me in other research or...
26 February 2020 9,330 2 View
Greetings, So, I am on the last phase of my project and the person who was supposed to look at my RNA seq data is currently busy thus won’t have time to touch it until next week. Hence, I was...
15 July 2019 7,456 4 View
I want heat source code in FORTRAN.
23 May 2016 1,009 1 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View
Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...
31 July 2024 3,893 0 View
Dear All, My lab primarily works on insect wing patterns. In one of the projects, my student and I have defined 19 abnormality characters on the forewing and 6 abnormality characters on the...
31 July 2024 6,464 5 View
Hello, I'm involved in a project focused on constructing and maintaining gravel roads in rural areas. We're particularly interested in ensuring durability and reducing maintenance costs over time....
29 July 2024 9,623 0 View
I have two groups that I test on three different tasks. I have 4 independent t-test, 6 paired t-test and 2 ANCOVA. My concern for which t-test should I conduct bonferroni correction. At the moment...
28 July 2024 7,827 6 View
Hey researchers! I am currently doing a research about to what extent in which A has accelerated the inclusiveness of the payment system in my country. Down below are a few examples of the...
26 July 2024 5,654 3 View
I am conducting a qualitative-driven approach to mixed-method research. The role of my quantitative data is to corroborate the findings of the qualitative data. Qualitative data has been collected...
24 July 2024 9,799 3 View
Hello! We are using LNP to deliver circular mRNA into T cells. However, compared with linear mRNA, the transfection efficiency and protein expression duration are similar. Are LNP generation or...
21 July 2024 4,322 1 View