30 May 2017 3 4K Report

 How long does it take to increase and to disappear acetate level in human serum after an intake of diet containing vinegar?

More Shirin Parvinroo's questions See All
Hello everyone, have anyone an experience about ligation with one digested vector(salI) with double digestion insert(SalI XhoI) that ligate together?

My vector is 7000 bps, and my insert is 1300 bps

28 May 2024 831 4 View

How can I optimize parameters of cyclic voltammetry to observe oxidation peaks?

I am investigating the redox behavior of Diene compounds by cyclic voltammetry in anaerobic environment. My electrodes include carbon glassy electrode as working electrode, platinum as counter and...

06 August 2023 8,463 3 View

How can we explain the expression changes between a total and phosphorylated form of a protein?

I am analyzing the expression of a receptor tyrosine kinases protein and its phosphorylated form via Western blots. The phosphorylated form has decreased significantly, and the total remains...

11 May 2023 9,655 9 View

Why there is no endogenous expression of my protein?

I tried to detect the endogenous expression of my protein of interest, but I can't. However, at the same time, I can verify the overexpression of the protein with different antibodies. I tried...

02 May 2023 3,547 4 View

DSP (cross-linking reagent) for suspension cells?

Hello, Has anyone ever used DSP ( a cross-linking reagent) for suspension cells before preparing cell lysate for performing co-Immunoprecipitation?

19 February 2023 2,223 0 View

Is there a solution to stain the bacteria and quench the dye and use another dye like DAPI to stain the cell?

In my project, I am trying to show Staphylococcus aureus in endothelial cells with fluorescent dyes. But when I used calcein AM, due to the presence of esterase enzyme in the bacteria and the...

28 October 2022 2,706 1 View

How to improve the concentration of input samples during immunoprecipitation?

Hello, I am doing co-immunoprecipitation to study the interaction of my protein of interest. The protein concentration after performing the Bradford assay for input samples is deficient. Does...

20 October 2022 2,267 8 View

How can I export voxel based file or tetra grid mesh from Avizo ?

I want to provide a voxel-based file or tetra grid mesh geometry for analysis in solvers like Abaqus. always generating tetra mesh face with different errors like the following figure. Does...

02 February 2022 2,090 2 View

How can I solve this error in Abaqus?

I exported the model from 3 matics to Abaqus for analysis but was faced with this error : 6766 elements have missing property definitions. The elements have been identified in element set...

24 January 2022 3,801 4 View

Simulating Px4 controller tool?

Hi everyone, I am trying to simulate Px4 controller, however I wanna change the drone dynamics. Is there any straightforward tool I can work with? The Matlab UAV toolbox's attitude controller is...

05 January 2022 8,126 4 View

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How am I supposed to find justifications for the unusual fatty acid profile in broiler diet?

I've obtained a fatty acid profile of broiler diet comprised of SFA 48 to 93%, MUFA (22 to 40%), PUFA (non determined to 10.4%. The PUFA composition is mainly n-6 and almost no value for n-3. The...

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Can type 2 diabetes be reversed at any age through proper diet and exercise?

Type 2 diabetes can be managed and, in some cases, put into remission through proper diet and exercise, regardless of age. Here are some key points: Weight Management: Achieving and maintaining a...

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Nutrition map: What does it mean for you?

Unluckily, just similar to an expensive car, your brain can be damaged if you ingest anything other than premium fuel.

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What are the signs and symptoms that can be observed in rats experiencing hypercholesterolemia?

Hi. I have been inducing hypercholesterolemia with a high-fat diet for 8 days, and what is happening to my rats is a decrease in their eating frequency, and their fur looks thinner and wet as if...

15 June 2024 1,746 3 View

Clarification on 3 x 2 factorial designs and reporting?

Hi, I am currently looking at a study where they used a 3 x 2 factorial design where they had 3 diets (A, B, C) and 2 enzyme supplementation levels (0 vs 1 %). Thus giving 6 treatments. Authors...

26 May 2024 4,626 1 View

Factorial Design Experiment Data in a Meta-Analysis?

Hi, I am currently creating a meta-analysis on the effect of growth performance of pigs in response to dietary fiber content in experimental diets as well as the influence of a fiber enzyme...

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Can i get desired publication?

"A high-fat diet promotes cancer progression by inducing gut microbiota–mediated leucine production and PMN-MDSC differentiation"

13 May 2024 1,321 1 View

Help me find the full text. I can only find the summary part in many databases, and I can't see the specific content. The author did not reply to me ?

Resveratrol supplementation or diet intervention effects on heart-liver axis in prediabetic female rats submitted to high-fat-high-sucrose diet

08 May 2024 9,891 1 View

Help me find the PDF, not only the abstract?

Resveratrol supplementation or diet intervention effects on heart-liver axis in prediabetic female rats submitted to high-fat-high-sucrose diet

05 May 2024 8,818 2 View

What is the good dilution factor for mouse serum in ELISA?

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