How long does it take for platelets to activate after washed platelet preparation if left at room temperature?

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Steingrimur Stefansson

Hi Rh Rj

. I would only keep washed platelets (serum free) at RT for 30 min at most with agitation (end-over-end slow mixing).

Luis A. Morán

, it depends on the platelet preparation methods that you use. I add PGI2 to prevent platelet aggregation during the centrifugation steps. PGI2 gets deactivated after 30 min. Therefore, I wait for at least 30 min (it is called resting) at RT on my bench, I do not agitate them during resting. Then you can start with the experiments.