How long does Calcein-AM staining last?

21 October 2021 0 2K Report

Extracting calcein-AM stained cell lines from mouse blood 13 days post injection. How likely are cells to still retain some signal after 13 days in blood?

Badges
Science topic

More Matt Mannino's questions See All

What is the purpose of Sephadex G-100 separation after PEG precipitation for exosomes/EVs in a publication?

Hello! I am reviewing a procedure for exosome/EV purification from a large-volume blood sample (porcine, terminal draw). In brief, protocol is: 1) Blood collection 2) Two-step spin to get...

26 October 2023 6,809 1 View

Anyone have any experience with Mac Elisa?

I am working to develop a Mac Elisa protocol that is able to capture the level of Immunoglobulin M (IgM) in mice infected with fungus T. marneffei. I've been mainly running into trouble with false...

04 September 2023 9,369 1 View

Post-synaptic currents or post-synaptic potentials?

Hello everyone, I would like some opinions regarding the measurement of synaptic transmission using patch-clamp electrophysiology. Specifically, when asking the broad question "do neurons from...

10 July 2023 2,932 3 View

Oxidation Peaks Sometimes at Positive Potential, Sometimes Negative (not a passivation issue)?

I have been performing redox sensing tests with bismuth oxychloride nanoflowers coated on a GCE for lead and zinc analytes in DI solution. The nanoflowers, mixed with Nafion, are applied to the...

27 April 2022 2,138 0 View

Can anyone recommend any publications on intersubjectivity in the context of education and/or learning?

I'm specifically interested in the application of intersubjectivity in the context of professional learning and teacher development. Thanks in advance Matt

26 January 2022 1,500 9 View

Looking for recommendations for using time-lapse camera traps to monitor and enumerate spawning salmon?

The goal of the project will be to use time-lapse camera traps (trail cameras) to monitor and enumerate salmon spawning past reference points in several streams. We will be aiming to identify when...

20 January 2022 7,306 3 View

Has anyone used Sloterdijk's Spheres (Foam in particular) to look at psychotherapy and counseling?

I'm curious if anyone has been thinking about how Sloterdijk's philosophies of space, ontology, and the psyche to work on psychotherapeutic practice, counseling, and/or making meaning. I see...

04 December 2021 4,084 1 View

Does anyone know of good vertical profiles or archaeological coss-sections through cemetery stratigraphy?

Cemeteries often remain in use over many generations, and may represent several hundred or even thousands of years of burial tradition. Some cemeteries in cities contain tens of thousands of...

15 November 2021 5,929 3 View

Can anyone recommend a good overview article comparing methods of sentiment analysis?

This isn't my specialty area, and I'm not in a position to conduct an extensive lit review, so I'm hoping you all can point me to your favorite sentiment analysis articles. By "sentiment analysis"...

19 September 2021 8,310 10 View

Should i report significance values or adjusted significance values when reporting pairwise comparison findings from a Kruskal-Wallis test?

This question has two parts (please explained in a simplified way as i am a very novice (and confused) user of SPSS) 1. Should i report significance values or adjusted significance values when...

04 August 2021 2,445 5 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View