Matt Mannino

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Questions related from Matt Mannino

RNase Inhibitor that is compatible with sequencing and qPCR?

Working on fine tuning mRNA isolation out of CTCs from blood. Tackling RNA degradation during the lysis and isolation step and we are considering RNase inhibitors to use in our reagents. Does...

09 June 2025 6,919 1 View

Is Glutamine addition to McCoy's Media necessary for Caki-2 & Caki-1 culturing?

I am preparing to work with Caki-1 and Caki-2 cell line cultures. I've seen a couple of biotech company websites suggesting the inclusion of 2 mM Glutamine to the McCoy's Modified Media that these...

16 October 2024 9,057 6 View

How long does Calcein-AM staining last?

Extracting calcein-AM stained cell lines from mouse blood 13 days post injection. How likely are cells to still retain some signal after 13 days in blood?

21 October 2021 2,024 0 View