I've heared phage can be stored in the long term adding glycerol.
How long can I use the phage after precipitation with PEG without adding glycerol in 4ºC?
I'm going to use precipitated phage to infect the bacterial cells for titer (input) and next round of panning.
Sibnarayan Datta
I dont know what kind of Phages you are working with, but in our lab, we have been using PEG precipitated Pseudomonas Phages stored in SM/TM buffer at 4C for more than one year. although there may be a slight drop in titer but, you can always enrich them again to get desired titers. However, please note that different phages may have different sensitivity to storage conditions.
best wishes,
SD
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Ziad Mitep Alkhozai
Hello yeo..
You can keep the phages for long time maybe for one yar
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