A cell free supeernatant of Lacttobacillus was prepared and stored in a refrigirator for 2-3 months to test its anticancer activity... is it still efective after this period of storing time or do I need to prepare a new one?
Yes, you can store culture for 3-4 months... But for any test you have to revive it
It depends on the nature of the metabolites present in the supernatant. Are proteins, organic acids or molecules of some other chemical nature. In any case, it is necessary to prepare a larger volume of supernatant, divide it into ependorfice and do a stability study, for example storage in the refrigerator at 2-8oC for 6 months. It is necessary to do a qualitative and quantitative analysis of the supernatant every (at least a month), with a obligatory anti-cancer activity test. This is the only way to be sure of the stability of the supernatant while storing the samples in the refrigerator.
My thanks and appreciation. Heenu Sharma Gordana Zavisic Mohamed Hassan
During storage the active compounds may be degraded into other inactive compounds
I prepared lactobacillus free supernatant and examined it as antimicrobil agent. The supernatant lost its activity after one week storage at 4 C . I think presence of proteases enzymes in the solution are responsible for decomposition of active ingredisnts
During storage the active compounds may be degraded into other inactive compounds
I prepared lactobacillus free supernatant and examined it as antimicrobil agent. The supernatant lost its activity after one week storage at 4 C . I think presence of proteases enzymes in the solution are responsible for decomposition of active compounds
During storage the active compounds may be degraded into other inactive compounds
I prepared lactobacillus free supernatant and examined it as antimicrobil agent. The supernatant lost its activity after one week storage at 4 C . I think presence of proteases enzymes in the solution are responsible for decomposition of active compounds
During storage the active compounds may be degraded into other inactive compounds
I prepared lactobacillus free supernatant and examined it as antimicrobil agent. The supernatant lost its activity after one week storage at 4 C . I think presence of proteases enzymes in the solution are responsible for decomposition of active compounds
During storage the active compounds may be degraded into other inactive compounds
I prepared lactobacillus free supernatant and examined it as antimicrobil agent. The supernatant lost its activity after one week storage at 4 C . I think presence of proteases enzymes in the solution are responsible for decomposition of active compounds
During storage the active compounds may be degraded into other inactive compounds
I prepared lactobacillus free supernatant and examined it as antimicrobil agent. The supernatant lost its activity after one week storage at 4 C . I think presence of proteases enzymes in the solution are responsible for decomposition of active compounds