How long do cells stay alive without defrosting them?
Almost indefinitely, if properly stored.
Thank you very much for your answer. Both cell lines are more than 1 year in liquid nitrogen. I hope that everythink will be OK with cells. Thanks
No problem, we routinely use cell lines that are way more than 1 year old. In the first week(s) after thawing if you notice a slow growth rate it could be useful to culture them in 20% FBS.
you can put your cells in liquid nitrogen for ever, but when you want thawing them try to keep them away from stress
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