I am investigating the apoptosis of T cells during dengue infection by detecting the cleaved caspase 3 in purified T cells isolated from infected spleen. I harvested the spleen 5 days post-infection but could not detect the caspase 3 in T cells. Is the infection period too long to catch the caspase 3?
Dear Fakhriedzwan!
Please You look a the following article:
Article Intrahepatic Infiltrating NK and CD8 T Cells Cause Liver Cel...
Asialo GM1+ cells reduced cleaved caspase 3 and TUNEL+ cells in the liver at day 1 after infection. CD8+ T cells infiltrated into the liver at later time point and at which time intrahepatic leukocytes (IHL) exhibited cytotoxicity against DENV-infected targets. Cleaved caspase 3 and TUNEL+ cells were diminished in mice with TCRβ deficiency and in those depleted of CD8+ T cells, respectively, at day 5 after infection.
https://www.nature.com/articles/s41598-017-14082-9
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