Under sealed conditions, the storage time may be long, but how many years is it?
Hi, I'm working on heart flow cytometry, and I use anti-mouse CCR2-BV785 (biolegend, cat150621) for staining, 20 minutes, 4 degree. But I couldn't see any CCR2+ cells. So I'm not sure if it's...
03 July 2024 3,969 4 View
Hi, I'm working on the immune cells in the heart and have a problem with CCR2 marker. I did some research and found there's CCR2+ monocytes in heart failure mouse model. When I analysed CCR2...
08 June 2024 7,315 5 View
Be curious and want to learn something from cases
20 May 2024 5,073 4 View
Hello, I am learning WRF-Chem, and there are some doubt I have not get the real answer. I used the chemical mechanism of MOSAIC for aerosol in WRF-Chem, where there is a variable called “oin” that...
17 May 2024 4,530 0 View
Hello, everyone! I got a very weird shape of volcano plot with R. It doesn't have a typical shape and I want to figure out the reasons behind this problem. In my plot, I found there were some...
10 May 2024 3,339 2 View
Hi guys, I'm working on the tumor mouse model. In general, I prepared tumor cells and injected cells into the flank of mice subcutaneously. After around 3 weeks, I found, in the same group,...
29 February 2024 9,034 3 View
Hi, I'm new to flow cytometry. Basically, I measured immune cells in the heart using the BD device. And I found sometimes the range of threshold rate was not similar among samples. For example, I...
21 November 2023 941 1 View
Hi, I'm working on the immunity of tumor. I injected tumor cells into the mice subcutaneously. After harvesting the tumor, I used percoll and isolated immune cells. Then I added trypan blue and...
20 November 2023 7,668 4 View
Hi there, I saw C57BL/6 was the most common mouse strain to be used as heart failure mouse model. I was just told that Balb/c was not suitable for heart disease, and acutally I don't know...
21 June 2023 364 3 View
As we know,AI is a hot topic in our society from the moment of its born, but we can't get more useful information about it,how does CHATGPT work?where can we get the entrance to use it? and where...
30 May 2023 8,540 6 View
Samuel Morse, the inventor of the Morse Code, understood that certain letters in the English language occurred more frequently than others (Gallistel and King 2010). To deal with this, Morse used...
01 August 2024 4,456 2 View
90% of museum objects worldwide are in storage.
21 July 2024 8,038 6 View
My study uses C57BL/6J mice and I am required to store portions of the important organs in neutral buffered formalin (NBF). Could I potentially keep those tissues in the formalin for a few weeks...
06 July 2024 8,360 2 View
What is meant by cross-contamination and what causes fruits and vegetables to become contaminated during preparation and storage?
02 July 2024 9,204 0 View
In a post-harvest handling chain (harvesting, transportation, storage), which among these do you think has the highest percentage loss why?
02 July 2024 7,649 0 View
I'm currently exploring the economic implications of solar PV curtailment versus the installation of battery energy storage systems (BESS). Specifically, I'm interested in recent publications or...
27 June 2024 6,423 2 View
Many studies have been conducted on Carbon Capture, Utilization, and Storage (CCUS) in oil fields to increase the recovery factor. Do you believe these studies are genuinely useful, or are they...
24 June 2024 7,441 0 View
We'll have vials in which we expect H2 generation to occur. Over the first few days, the amount of generated H2 is expected to be at ppb level. GC (+ TCD) detector is best when your H2 level is...
18 June 2024 254 3 View
I need to analyse proximate composition in whole fish and antioxidant enzyme analysis. What is the protocol to store these samples for maximum efficiency in the results. I have -80 degree C...
05 June 2024 1,372 6 View
explain the chemical properties and dielectric properties of homemade almond milk.
26 May 2024 5,938 0 View