I am carrying out DPPH free radical assay to determine antioxidant potential of plant extracts, if I wish to use that solution on the second day and so on; till how many days it can be used further?
i would recommned you to do fresh soln every time when you r going to set your experiment but for just try and and check the compatibity of compund whether it is antioxidant or not you can you it sa per ider soufian say. i find the same problam when i use previous stock soln of DPPH to carry my experiment next day. you will find less absorbance in control soln.