Does anyone know the ideal cell density for establishing TIB-75 xenograft in BALBc mice?

I am trying to establish TIB-75 cell line xenograft in BALBc mice.

19 January 2021 5,813 2 View

QPCR considerations in analyzing samples from the field?

I am attempting to plan a qPCR experiment in which I evaluate the expression of multiple genes in various brain regions of fetuses, as a function of location in utero (intrauterine position,...

31 October 2020 5,788 1 View

Can I perform immunohistochemistry on tissue after it has already been flash frozen?

I did not plan on staining but now it is a necessity. So tissue was immediately frozen using liquid nitrogen and then placed in -80 C after removal. This is human tissue and I would like to stain...

08 October 2017 4,357 3 View

What is the best way to evaluate that the AZD-7762 inhibitor is working on Chk1 upon DNA damage?

Hi! I am trying to inhibit Chk1 kinase phosporilation upon DNA damage. I was thinking on using the inhibitor AZD-7762. What is the best way to evaluate that the AZD is working? Should a western...

05 January 2016 1,464 3 View

Any advice for total protein quantification from cells suspended in FBS?

11 December 2015 9,041 3 View

Why is there no separation in PFGE?

I tried to use PFGE system CHEF II (Bio-Rad), but even on length marker (also Bio-Rad) I cannot receive any DNA separation. I used 0,5X TBE buffer, certified molecular biology agarose from...

13 May 2015 4,614 14 View

What are the best data mining tools for health care data?

We have moved into the era of "big data", and tools that have traditionally been applied to other industries are now being considered in health care. Data sources include claims data, survey data,...

24 March 2015 3,170 11 View

Are there any methods to isolate microorganism which can not grow on standard agar plates?

Hi, I'm lookong for methods for the isolation of microorganisms from liquid consortium, which could not grow on standard, solidified agar. Strains which grow on agar plates are unable to degrade...

17 November 2014 2,572 10 View

Why am I getting no PCR products in long range PCRs using cDNA as template?

08 June 2014 3,011 14 View

Looking for laws and regulations addressing ecologic noise pollution?

01 January 1970 9,401 4 View

Physics: Why do we describe light by referring to its wavelength, but microwaves and radar pulses by referring to their frequency?

In physics, the description of the properties of light generally refers to its wavelength (nm), while in the range of microwaves and radio waves we refer to the frequency of a signal (e.g. kHz or...

03 March 2021 1,682 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

Where can I find atmospheric measurement profiles (temperature, density, etc.) between 300-800 km altitude?

I am investigating thermospheric mass density profiles between 300-800 km altitude. I would like to compare the variability with other parameters, including temperature, electron content,...

01 March 2021 5,902 3 View

Could anyone tell me what is the range of submillimeter wave?

There is confusion regarding the submillimeter-wave range. Many papers specify its range from 300GHz but some also consider its range from 100GHz. If I design an antenna on 122ghz what would I...

01 March 2021 516 7 View

Is there a deep reason for using the squared modulus of the wave function as the probability density of the existence of the particle?

I think we were all surprised at the first time we got to know quantum mechanics that the squared modulus of the wave function is the probability density of the existence of the particle? The role...

28 February 2021 6,282 9 View

What is Flat Lens Antenna?

28 February 2021 5,433 3 View

Complex wave form in ANSYS?

Is there a way to input wave speed in a complex form on ANSYS? The wave speed I need to assign a material is 325(1+0.11i) however I have been unable to do this thus far. Failing that, is there...

27 February 2021 5,969 1 View