The appropriate treatment may vary depending on the species and variety. In a study on two selected varieties of Calathea crotalifera, the highest number of multiple shoots was obtained in MS medium supplemented with 3.5 mg/L BAP, 1.0 mg/L NAA, 3% sucrose, and 6 g/L plant agar for both varieties. The maximum roots induction was recorded on MS medium supplemented with 1.0 mg/L NAA. In C. ornata cv. Sanderiana, the highest number of shoots was obtained on MS medium containing 2.5 mg/L BAP, 2.5 mg/L Kin, 4.5 % sucrose.