I used an 1,5% agarose gel for a second time and I loaded in the wells that I never used 15 microlitres of sample (constituted of 3 microlitres of bromophenol blue 6X, 6 microlitres of sample and 6 microlitres of water). I set the run at 120 V. The run started, but the major part of bromophenol blue remained in the well except a little part. So I couldn't stop in time the run, because the small percentage of blue bromophenol then became invisible.
Are you getting plenty of bubbles from the electrodes. If the power supply is not working or one electrode is broken then you may simply not have current through the gel and buffer? The small amount that moves may just be diffusion. Check the electrode connections and run just buffer to check for bubbles and see what the current being delivered is.
Hello
Making a 6X Bromophenol blue needs a very accurate digital scale. Maybe the concentration of your solution is more than 6X.
Concentration shouldn't matter. Agree with Paul Rutland. Sounds like your problem is electrical. If the dye stays in the well that's an indication that the current isn't high enough to run it through. Either your power supply is not working properly or you're having some other current issue.
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