Paola Zanfardino

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Questions related from Paola Zanfardino

Why does bromophenol blue band seem to split up during this SDS PAGE run?

Samples (constituted by Laemmli buffer 4X, total protein extract and water) were loaded after denaturation at 70 ° for 10 min. Bromophenol blue reformed an unique band only towards the end of the gel

07 January 2021 7,921 4 View

How is possible that only a small percentage of bromophenol blue started the run in agarose electrophoresis ?

I used an 1,5% agarose gel for a second time and I loaded in the wells that I never used 15 microlitres of sample (constituted of 3 microlitres of bromophenol blue 6X, 6 microlitres of sample and...

08 December 2020 1,503 3 View