Can a random-intercept cross lagged panel model (RI-CLPM) be indentified with just two measurement points?

I am currently planning out my masters' thesis design and will be working with a large data set with variables that have been meseasured at two time points. Intially I thought of just using a...

21 January 2021 9,384 4 View

Why fouled membrane has more negative zeta potential and lower IP?

I modified PES membranes with nanoparticles (nanosilver, graphene, halloysite). They were used in UF for WWTP effluent treatment. After filtration, all membranes exhibited more negative zeta...

26 December 2020 5,676 3 View

Will making a hydride acceptor more electrophilic speed up hydride transfer?

I have been searching the literature about this and I have not found an answer. If the atom that accepts a hydride is made more electrophilic, can this speed up the rate of hydride transfer?

29 July 2020 9,944 1 View

Can the energy displayed in Gaussian Calculation Summary be used to compare the stability between molecules?

I want to compare the stability of molecules that have the same number of atoms. I optimized these structures and calculated their vibrational frequencies. Gaussian09 gives a Gaussian Calculation...

18 July 2020 5,243 5 View

Could anyone suggest some articles and reliable sources about the (expensive) price of ruthenium and its complexes fabrication?

I'm writing and article about dye sensitized solar cells and I need a reliable source (articles, journals, books, reviews...) to base my statement (that ruthenium itself and ruthenium complexes...

07 February 2020 8,172 1 View

Can I use different rotation tecniques to compare extract factors in different years?

Dear researchers, I obtained a rotation matrix (varimax) in my factor analysis with correlation no higher than 0.20 for my 2010 data. However, for my 2014 data, with varimax rotation, I obtained...

20 January 2020 5,393 2 View

Do you know of any (ideally German) open source intelligence measures?

We are planning a study, in which we want to measure verbal, numerical, and spatial intelligence in university students. The study takes place in a German-speaking country and we had originally...

19 August 2019 175 4 View

Which temperature should I use in Molecular dynamics (GROMACS) for a protein found in endoplasmatic reticulum?

Hi everyone, I'm trying to perform a MD simulation for a protein found in ER. In mdp file should I set: ref-temp= 300 K or 310K? gen-temp should be the same than ref-temp or 300...

22 June 2019 2,083 6 View

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I'm developing a MethyLight assay to verify a specific CpG site in a promoter region of my interest gene in rats. Unfortunately, I don't have a lot of choices to design the probe and primers,...

20 June 2019 1,004 3 View

How to compare two RMSD plot (frame vs frame) from gromacs?

I have two proteins. One is wild-type and the other is a mutant. The RMSD plot (nm vs time) showed me mutant is far more unstable. But, when a plot the RMSD frame vs frame graphic it seems mutant...

28 May 2019 7,260 7 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Could anyone tell me what is the range of submillimeter wave?

There is confusion regarding the submillimeter-wave range. Many papers specify its range from 300GHz but some also consider its range from 100GHz. If I design an antenna on 122ghz what would I...

01 March 2021 516 7 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Is there a deep reason for using the squared modulus of the wave function as the probability density of the existence of the particle?

I think we were all surprised at the first time we got to know quantum mechanics that the squared modulus of the wave function is the probability density of the existence of the particle? The role...

28 February 2021 6,282 9 View