11 May 2020 1 2K Report

I am getting negative CRR values while determining liquefaction potential using shear wave velocity approach .

More Gurpreet Singh Bhatia's questions See All
Can anyone recommend an RNA extraction protocol from Cacao tissue (leaves, meristem, branch)?

Due Covid-19 I personally cannot visit a rural site for the collection of samples. But have found some great people who are happy to do the extractions on my behalf. I need to provide them with an...

19 November 2020 4,049 3 View

How to plot upper bound and lower bound limit curves of trendline in MS Excel?

To obtain upper and lower bound curves for trendline of scattered plot.

10 May 2020 2,117 1 View

How can I design allele specific primers when I have twp SNPs only few base pairs apart?

Hi there, I am trying to design allele specific primers for KASP assays to validate the SNPs from GWAS results. However, few of my SNP sequences have two or more SNPs only few base pairs apart and...

13 August 2019 9,759 4 View

How can I prevent the degradation of the DNA?

I have extracted DNA from cacao leaf tissue (freeze dried). I used CTAB buffer (5%) along with 1% B-mercaptoethanol and 3% PVP. I did the DNA extraction overseas and transported it as a pellet to...

16 January 2019 2,055 12 View

Why am not seeing any DNA bands from my samples?

I have extracted DNA from Cacao leaf tissue using CTAB, chloroform isoamyl alcohol method. When I quantified my samples on Nandodrop they show a conc. of 300-800 (ng/ul) in 50 ul of TE buffer. The...

15 January 2019 2,144 14 View

Is it okay If I donot remove RNAse from my DNA samples after treating it for an hour at 37 C?

I am trying to remove RNA contamination from my DNA samples. I gave RNAse treatment for DNA resuspended in 50 ul of TE buffer. After 1 hour of treatment I reprecipitated the DNA using 1/10 V of...

14 January 2019 5,817 2 View

Does keeping tissue sample (patient biopsies) in Trizol for a long time (>6 month) degrades DNA ? or Leads to sheared DNA?

I am trying to isolate RNA and DNA from the tissue biopsy stored in Trizol at -80 degree for more than 6 months . I isolated RNA using the standard Trizol method and obtained good quality and...

22 November 2018 2,446 2 View

Why are my gel pieces not getting destained for in-gel digestion?

My gel pieces are not getting destained. I have kept it for more than 6 hours now. I m using 50mM Ammonium bicarbonate and Acetonitrile in ratio of 7:3.

07 May 2018 5,419 1 View

How to properly store the sds gel pieces for in gel digestion?

I have already cut the gel pieces and would like to perform in gel digestion in future. So I had freeze them in 1% acetic acid. Can I still process those samples?

15 January 2018 1,120 1 View

Is proteomic data analysis in R (using RforProteomics) better than any other proteomic software?

My project involves analyzing proteome of my sample. I have more than 100 samples for analysis. Would proteomic analysis in R help me? Is it better than other softwares?

04 January 2018 2,591 6 View

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