I am interested in understanding how the surface area changes when adherent cells are brought into suspensions. I am looking for data supported responses.
No one is interested in measuring the surface area in both attached and suspended cells. Due to tremendous change of cell niche in both conditions, stress, cytoskeletal system, metabolic events, the resulting signaling system received within the cell will be totally different. Therefore, it is pointless to discuss, so, no papers. Just imagine monomeric actin molecules and dimeric tubulin molecules either polymerize or depolymerize in a brink of time within the cells. This cell movement accompanies with different metabolic activities. The membranes are not a fixed boundary, and endomembane system is always flowing from the plasma membranes, cis-, medial-, and trans-Golgi membranes, and ER membranes to the nuclear membranes and ever-changing. Full appreciation of these basic biological systems can only tell that there is no surface area change in both states of cells, but changes occur in cytoplasm. Just like a completely air-leaked balloon and maximally inflated balloon!!! Still doubt?
You may be interested in the followings;
Cell 16 (4), 909-918 (1979)
Biotechnol Prog 9 (4), 362-365 (1993)
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