I want to analyse my silver nano-particle samples by dynamic light scattering so for that I did't find any online protocol or procedure to prepare samples for DLS zeta potential analysis so anyone there who can help me in that.
Your question is confused - are you measuring size or zeta potential or both?
Typically (if these are genuinely nano i.e. < 100 nm), no sample preparation is required for a 173 degree (back scatter) system. No parameters are required for the intensity distribution other than the RI of the continuous phase (usually water at 1.33). If the sample is highly concentrated then any dilution must take place in the mother liquor.
Just put the sample in the appropriate cuvette and measure away.
For sizing, make sure you have at least 10-5M salt. If you don't and there is a high zeta potential, you may get overestimated size. This is due to a phenomenon called electrohydrodynamic retardation.
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