First you need to run control against all samples. The data I used to put in to excel are the "absolute gene regulation" and the "standard error" for each sample. Control is equal 1. Gene expression 2 does not mean 2 fold expression. The cT value is transformed, than compared. Take a look in to the Pfaffl (2002) paper for more details. If you need any thing else, just let me know.

Sabine